Under what circumstances will the chromogenic substrate TOOS undergo oxidation and deterioration

In the fields of biochemical testing and medical diagnosis, the chromogenic substrate TOOS (N-ethyl-N - (2-hydroxy-3-sulfopropyl) -3-methylaniline sodium salt) is an important reagent widely used in the detection of blood glucose, uric acid, liver function, and other items. However, TOOS may undergo oxidative degradation under certain conditions, which can affect the accuracy and reliability of its detection results. This article will explore in detail the causes, influencing factors, and preventive measures of TOOS oxidation deterioration.

Reasons for oxidation and deterioration of TOOS

The oxidative deterioration of TOOS is mainly due to its reaction with oxygen or other oxidizing substances in the air, resulting in a change in chemical structure and the loss of its original activity and color development ability. Specifically, the oxidative degradation of TOOS may be influenced by the following factors:

1. Oxygen in the air: TOOS has reducibility, and prolonged exposure to air can cause it to react with oxygen, leading to oxidation and deterioration. This oxidation reaction will alter the chemical properties of TOOS, causing it to lose its original color rendering ability.

2. Temperature: High temperatures accelerate the rate of chemical reactions, including oxidation reactions. If TOOS is exposed to high temperatures during storage or use, it will accelerate its oxidation and deterioration process. Therefore, storage temperature is an important factor affecting the stability of TOOS.

3. Humidity: Excessive humidity may cause TOOS to absorb moisture from the air, form hydrates, or undergo other chemical reactions, thereby affecting its stability and activity.

4. Light exposure: Prolonged exposure to light may cause photochemical reactions in TOOS, producing free radicals or other active substances, which can lead to oxidative degradation.

5. Impurity contamination: If TOOS is contaminated with oxidative impurities during preparation or storage, these impurities may accelerate its oxidation and deterioration process. Therefore, the purity of raw materials and the cleanliness during preparation and storage are crucial for the stability of TOOS.

Factors affecting TOOS oxidation deterioration

In addition to the direct causes mentioned above, the oxidative deterioration of TOOS may also be influenced by the following factors:

1. Material of storage container: Storage containers of different materials may have different effects on the stability of TOOS. For example, certain plastic containers may release harmful substances that react with TOOS, leading to oxidative deterioration.

2. Storage time: Long term storage may cause TOOS to gradually lose activity, and even under good storage conditions, oxidation and deterioration may occur due to time factors.

3. Usage: When using TOOS, improper operation, such as not preparing the solution according to the specified ratio in the manual, not fully mixing, etc., may also affect its stability and color development effect.

Measures to prevent TOOS oxidation and deterioration

To extend the shelf life of TOOS and maintain its activity, the following preventive measures can be taken:

1. Storage conditions: Store TOOS in a low temperature, dry, and dark environment, such as a refrigerator (recommended temperature is 2-8 ° C). Avoid prolonged exposure to high temperatures, high humidity, or light conditions.

2. Storage container: Choose a storage container with stable material and no harmful substance release, such as glass bottles or stainless steel containers. Avoid using plastic containers that may release harmful substances.

3. Ready to use: When using TOOS, the principle of ready to use should be followed to avoid color changes caused by prolonged storage. The prepared solution should be used up as soon as possible to avoid prolonged storage.

4. Raw material purity: When preparing TOOS, the purity of the raw materials should be ensured and the use of raw materials containing oxidative impurities should be avoided. At the same time, cleanliness should be maintained during preparation and storage to avoid contamination.

5. Operating standards: When using TOOS, it should be strictly prepared and used according to the proportions and operating steps specified in the instructions. Ensure that the solution is thoroughly mixed to avoid errors caused by improper operation.

Conclusion

The chromogenic substrate TOOS has wide applications in biochemical detection and medical diagnosis, but its oxidative degradation problem cannot be ignored. By understanding the causes, influencing factors, and preventive measures of TOOS oxidation deterioration, we can better protect its stability and activity, thereby improving the accuracy and reliability of detection. In practical applications, we should strictly adhere to storage and usage standards to ensure that the quality and performance of TOOS are fully utilized. Meanwhile, with the continuous advancement of science and technology and the emergence of new chromogenic substrates, we should actively explore and apply more stable and sensitive chromogenic substrates to meet the needs of clinical testing and scientific research.

Desheng specializes in producing more than ten the new Trinder's reagents, including TOOS. After more than ten years of research and development, it can ensure that TOOS appears as a powder with a purity of up to 99.5%, strong water solubility, and stable performance to ensure the accuracy of experimental results. Desheng has a place in the market for in vitro diagnostic kit raw materials with high-quality products, and is deeply trusted and supported by customers at home and abroad. If you have any relevant intentions, please click on the official website for consultation!