Tris is a zwitterionic buffer, often used in biology and scientific research experiments, of course, there are also many directly used in medicine and industry. So what are the specific uses of the different buffers of tris, how to use the buffers of tris, and understand the characteristics of tris and different biotechnologies, such as electrophoresis or chromatography.
Basic information of TRIS base:
CAS number: 77-86-1
Molecular weight: 121.14 g / mol
Formula: C 4 H 11 NO 3
Available pH range: 7.2-9.0
pKa (25°C): 8.06
Maximum wavelength (λmax)λ: 260 nm Amax: 0.10: λ: 280 nm Amax: 0.08
What is TRIS recommended for?
lCommonly used in gel electrophoresis buffer (TAE or TBE)
lUsually used to prepare Laemmli buffer (used for denaturation and loading of protein samples in SDS-PAGE)
lAnion exchange chromatography
lUsed to evaluate bacterial endotoxin
lFor capillary electrochromatography (CEC), due to its low ion mobility
lUsed for metal complexation
l Can be used to correct acidosis
What issues should be considered before choosing TRIS for research?
uNot very effective below pH 7.5
uIt has potentially reactive primary amines and can usually be used as an inhibitor
uIt tends to permeate the membrane, so it is not suitable for most cell culture work
uToxic to many mammalian cells
uIt is not suitable for bicinchoninic acid (BCA) analysis
uIt may interfere with the Bradford dye binding method for protein determination
uChelated calcium and other essential metals are well known
uThe pH of the solution containing Tris is very dependent on temperature, so it is very important to prepare Tris buffer at the temperature of use
uIt may interact with the bicarbonate/CO2 buffer system
According to the use of different industries, the considerations are different, and the requirements for the level of tris will also be different, the purity and some other indicators will also be relatively different, and these factors often require buyers and manufacturers to be able to deepen Communication can be better provided.
Tips about TRIS:
ØHigh solubility in organic solvents
ØIt reacts with various enzymes and inhibits several enzymes
Ø It can inactivate the popular RNase inhibitor DEPC
