Tris HCl, abbreviated as Tris HCl, is an organic compound widely used in the fields of biochemistry, molecular biology, and chemical engineering. Its chemical formula is C4H11NO3 · HCl, CAS number is 1185-53-1, molecular weight is 157.60, and it usually exists in the form of a white powder. Tris (hydroxymethyl) aminomethane hydrochloride plays an irreplaceable role in various biochemical experiments due to its buffering properties and stability.
1、 Basic properties and structure
Tris hydrochloride is formed by reacting Tris with hydrochloric acid. It retains the buffering properties of Tris and adjusts the pH value by adding hydrochloric acid, enhancing its stability in acidic environments. Tris itself is an organic alkaline compound with three hydroxymethyl groups and one amino group, which endow it with strong buffering ability. When combined with hydrochloric acid, the pH range of trihydroxymethylaminomethane hydrochloride is usually between 7.0-9.0, which is suitable for various biological experimental conditions.
2、 Physical and chemical properties
Tris (hydroxymethyl) aminomethane hydrochloride has good water solubility and can quickly dissolve in water to form a clear solution. Its molecular weight is 157.6, which ensures its good solubility in solution and avoids the decrease in bioavailability caused by excessive molecular weight. In addition, the purity of trihydroxymethylaminomethane hydrochloride is high, reaching over 99%, which provides a guarantee for its stable performance in biochemical experiments. The appearance of its white crystals also reflects the control of impurities during its production process.
3、 Buffer Performance and Applications
Tris (hydroxymethyl) aminomethane hydrochloride plays a crucial role as a neutral buffer in various biochemical experiments. Its buffering range (7.0-9.0) covers the pH values required for most biological experiments, which can maintain the acid-base balance of the reaction system and avoid the influence of pH fluctuations on experimental results. In experiments such as protein electrophoresis, enzyme activity determination, and nucleic acid electrophoresis, trihydroxymethyl aminomethane hydrochloride is widely used as a buffer solution. In addition, it can also be used for protein purification, crystallization, and cell culture processes, providing great convenience for biochemical experiments.
The buffering capacity of trihydroxymethylaminomethane hydrochloride mainly comes from the amino and hydroxyl groups on its molecule. These functional groups can absorb or release hydrogen ions, thereby maintaining the pH stability of the solution. When the solution is affected by acidic or alkaline substances, trihydroxymethylaminomethane hydrochloride can quickly adjust the pH value of the solution through its buffering effect, maintaining the stability of the experimental environment.
4、 Stability and Storage
Tris (hydroxymethyl) aminomethane hydrochloride has good stability and can be stored for a long time at room temperature and pressure without significant decomposition or deterioration. However, to ensure its performance is not affected, it is recommended to take appropriate measures during storage and transportation, such as sealing, storing in a cool and dry place, avoiding direct sunlight and high temperature environments. For long-term storage solutions, it is recommended to store them below -4 ℃. However, in practical use, in order to ensure the stability of the product, researchers usually recommend on-site preparation of trihydroxymethylaminomethane hydrochloride buffer solution.
5、 Preparation and use
When preparing the buffer solution of trihydroxymethylaminomethane hydrochloride, it is necessary to choose appropriate raw materials and pH values. High purity trihydroxymethylaminomethane hydrochloride raw material should be selected to ensure the accuracy of experimental results. Secondly, determine the required pH value and concentration based on experimental requirements, and determine the amount of raw materials to be added through calculation. During the preparation process, add an appropriate amount of trihydroxymethylaminomethane hydrochloride powder to deionized water, stir thoroughly to dissolve it completely, and adjust the pH to the desired value by adding an appropriate amount of hydrochloric acid or sodium hydroxide. The prepared buffer solution needs to be filtered to remove impurities and particles, and stored in a sealed container to avoid direct sunlight and high temperatures.
Tris (hydroxymethyl) aminomethane hydrochloride, as an important biochemical reagent, has a wide range of applications in the fields of biochemistry, molecular biology, and chemical engineering. Its buffering performance and stability provide reliable guarantees for various biochemical experiments. During use, researchers should pay attention to performance indicators such as purity, solubility, buffering range, and stability to ensure the accuracy and reliability of experimental results. As an advantageous manufacturer of biological buffering agents such as trihydroxymethylaminomethane hydrochloride, Desheng can supply raw materials with a purity of 99% for manufacturers to prepare and use, which is convenient, simple, and has stable buffering performance. If you have any relevant intentions, please feel free to inquire for details!
