Application of TOOS reagent in enzymatic spectrophotometric determination of hydrogen peroxide

In the fields of biochemistry and medical diagnosis, hydrogen peroxide (H ₂ O ₂) is an important reactive oxygen species molecule, and accurate determination of its concentration is of great significance for monitoring various biochemical reactions, disease diagnosis, and evaluating treatment effectiveness. With the advancement of technology, enzymatic photometry has gradually become the preferred method for detecting hydrogen peroxide due to its high sensitivity and easy operation. In this method, N-ethyl-N - (2-hydroxy-3-sulfopropyl) -3-methylaniline sodium salt (TOOS), as a novel Trinder's reagent, has brought new breakthroughs to the determination of hydrogen peroxide with its unique advantages and wide applicability.

Innovations in TOOS reagents

Although traditional Trinder's reagents have been widely used in hydrogen peroxide determination, they have problems such as poor water solubility, insufficient stability, and susceptibility to environmental interference, which limit their application in certain complex samples or special conditions. In contrast, TOOS reagents have innovated in their chemical structure by introducing specific functional groups, significantly improving their water solubility and enabling more uniform dispersion in solution, reducing errors caused by precipitation. In addition, TOOS also exhibits a wider pH adaptability range, which means that it can maintain stable reaction performance under different biological samples and experimental conditions, which is crucial for improving the accuracy and repeatability of detection.

The mechanism of enzymatic photometric determination of hydrogen peroxide

Enzymatic photometry is a method for determining the concentration of target molecules based on the color changes produced by enzymatic reactions. In the determination of hydrogen peroxide, peroxidase (such as horseradish peroxidase) is usually used as a catalyst, which can promote the oxidative coupling reaction between hydrogen peroxide and TOOS and another auxiliary substrate (such as 4-aminoantipyrine or 3-methylbenzothiazole sulfone). This reaction produces a dye with a strong color (usually purple or blue), whose color depth is proportional to the concentration of hydrogen peroxide. By measuring the absorbance of this dye, the concentration of hydrogen peroxide can be indirectly calculated.

Advantages and Applications of TOOS Reagents

The advantage of TOOS reagent in enzymatic photometric determination of hydrogen peroxide is not only reflected in its improved chemical properties, but also in its versatility and convenience in practical applications. Firstly, its high water solubility makes preparation and use more convenient, reducing errors caused by insufficient dissolution. Secondly, the wide pH range allows TOOS reagents to be applicable to different types of biological samples, such as blood, urine, tissue homogenates, etc., without the need for complex preprocessing of the samples, thereby improving detection efficiency.

Furthermore, due to the good stability of the dye generated by the reaction between TOOS and hydrogen peroxide, which is not easily affected by factors such as light and temperature, it can maintain color stability for a long time, making it easy to read and record the results.

In the field of medical diagnosis, the application of TOOS reagents is particularly widespread. For example, in blood glucose detection, by monitoring the amount of hydrogen peroxide produced by glucose oxidation catalyzed by glucose oxidase, the blood glucose concentration can be calculated indirectly, providing an important basis for the diagnosis and treatment of diabetes. In addition, in routine liver function tests, the concentration changes of hydrogen peroxide are closely related to the redox status of the liver, and the degree of liver damage can be evaluated by measuring its content. In blood lipid testing projects, such as the determination of triglycerides and cholesterol, hydrogen peroxide is a key intermediate product in the reaction process, and accurate determination of its concentration is of great significance for evaluating the risk of cardiovascular disease.

Summary

In summary, TOOS reagent, as a novel Trinder's reagent for enzymatic spectrophotometric determination of hydrogen peroxide, has brought important innovation and development to the fields of biochemistry and medical diagnosis with its unique advantages and wide application areas. With the continuous advancement of technology and the deepening of applications, we believe that TOOS reagents will play a more important role in the future and make greater contributions to human health.

Desheng specializes in producing more than ten the new Trinder's reagents, including TOOS. After more than ten years of research and development, it can ensure that TOOS appears as a powder with a purity of up to 99.5%, strong water solubility, and stable performance to ensure the accuracy of experimental results. Desheng has a place in the market for in vitro diagnostic kit raw materials with high-quality products, and is deeply trusted and supported by customers at home and abroad. If you have any relevant intentions, please click on the official website for consultation!