Wuhan Desheng Biochemical Technology Co., Ltd

Enzymes play a crucial role as biocatalysts in the field of biochemistry, enabling efficient execution of numerous chemical reactions. However, the properties of enzymes are relatively fragile, and their activity is susceptible to interference from various physical and chemical factors. It is crucial to have a deep understanding of these influencing factors and take appropriate measures to fully utilize the role of enzymes in practical applications. Temperature impact and response strategies Temperature is an important physical factor that affects enzyme activity. Within an appropriate temperature range, enzyme molecules can maintain a specific spatial structure, effectively bind to substrates, and catalyze reactions. But when the temperature exceeds 60 ℃, the enzyme activity will rapidly and irreversibly decrease. This is because high temperatures can break the chemical bonds within enzyme molecules, causing changes in their spatial structure and resulting in the enzyme losing its catalytic function. In order to protect enzyme activity, high temperature environments should be avoided when storing enzyme preparations, and they should be stored at low temperatures of 4-8 ℃. This can effectively slow down the thermal movement of enzyme molecules and maintain their structural stability. The role and adjustment methods of pH value The pH value of the solution has a significant impact on enzyme activity. The functional groups on the surface of enzyme molecules exhibit different charge states at different pH environments, which can affect the binding ability between the enzyme and the substrate. Under extreme pH conditions, enzymes may undergo precipitation, but this inactivation can be restored under certain conditions. When the pH value returns to the appropriate range for the enzyme, the enzyme molecules can dissolve again and partially restore their activity. To maintain the stability of enzyme activity, using specialized buffer to adjust the pH value of the solution is an effective method. Buffer solution can resist the addition of external acidic and alkaline substances within a certain range, maintain a relatively constant pH value of the solution, and provide a stable working environment for enzymes. Key points for handling shear force and salt ion concentration Shear force is also one of the factors that affect enzyme activity. The strong shear force generated by vigorous stirring and other operations can directly damage the structure of enzyme molecules, leading to enzyme denaturation and inactivation. Therefore, when performing operations involving enzyme reconstitution, vigorous stirring should be avoided as much as possible, and a gentle mixing method should be used to reduce mechanical damage to enzyme molecules. In addition, the concentration of salt ions is equally important for the stability of enzymes. When storing or dissolving enzymes, they need to be placed in a buffer solution because the salt ions in the buffer can maintain a certain ionic strength, ensuring the stability of the enzyme molecular structure. Directly dissolving enzymes in pure water may alter the ionic environment surrounding the enzyme molecules, affecting their structure and activity. The stability of enzyme activity is crucial for its use in biochemical research and practical applications. Factors such as temperature, pH value, shear force, and salt ion concentration can all have varying degrees of impact on enzyme activity. By adopting appropriate storage and handling methods, such as controlling temperature, adjusting pH with buffer solution, gentle handling, and avoiding pure water dissolution, the stability of enzyme activity can be effectively maintained, thereby better utilizing the catalytic properties of enzymes and promoting the development and application of biochemistry. Hubei Xindesheng Material Technology Co., Ltd. has established a professional enzyme preparation research and development team to develop high-quality and stable enzyme preparation products. The professional technical team can answer any questions you may have during the use and storage of enzyme preparations at any time. If you have any recent purchasing needs, please click on the official website to learn more details or contact me directly!  

In the field of chemical and biological detection, acridine ester DMAE-NHS is like a shining star, playing a key role in chemiluminescence immunoassay and other areas due to its unique optical properties. However, its thermal stability is influenced by multiple factors, and understanding these factors is crucial for fully unleashing its performance. Temperature and pH: A double-edged sword of thermal stability The thermal stability of acridine ester DMAE-NHS is not constant, but decreases with increasing pH and temperature. This characteristic is like a double-edged sword, exhibiting completely different states under different conditions. At room temperature, DMAE-NHS exhibits relatively stable characteristics in PB buffer solutions with pH values of 5.8, 7.0, and 8.0. This means that under milder conditions, it can maintain its chemical structure well, thereby maintaining its luminescent activity. However, this stability is not absolute, and over time, its luminescent activity will still decrease to a certain extent. When the temperature rises to 37 ℃, there is a significant change in the situation. In PB buffer solutions with pH values of 5.8, 7.0, and 8.0, the decrease in luminescent activity of DMAE-NHS significantly increased after 16 days. This indicates that the increase in temperature has a significant negative impact on the thermal stability of DMAE-NHS. Temperature acts as a catalyst, accelerating the movement and changes within molecules, making DMAE-NHS more susceptible to reactions that are detrimental to its luminescent activity. The change in pH value cannot be ignored either. At the same temperature, as the pH value gradually increases from 5.8 to 8.0, the luminescence activity of DMAE-NHS decreases more and more. This indicates that alkaline environments are more detrimental to the thermal stability of DMAE-NHS. Under alkaline conditions, the concentration of hydroxide ions in the solution increases, providing more favorable conditions for the hydrolysis reaction of DMAE-NHS, thereby accelerating the loss of its luminescent activity. Hydrolysis: the 'mastermind behind the decrease in thermal stability' Behind the decrease in luminescent activity of DMAE-NHS in PB buffer over time, there is a key factor hidden - hydrolysis. The hydrolysis reaction is like an invisible 'killer', quietly disrupting the chemical structure of DMAE-NHS. In alkaline environments, hydrolysis reactions receive "assistance". Under alkaline conditions, water molecules are more likely to ionize into hydroxide ions, which attack specific chemical bonds in DMAE-NHS molecules, causing them to break and trigger hydrolysis reactions. As the hydrolysis reaction proceeds, the molecular structure of DMAE-NHS changes, and the parts that could have produced luminescent effects are destroyed, resulting in a natural decrease in luminescent activity. Raising the temperature will also contribute to the hydrolysis reaction. The increase in temperature intensifies the thermal motion of molecules, leading to an increase in the collision frequency and force between DMAE-NHS molecules and water molecules. This provides more energy and opportunities for hydrolysis reactions, making them easier and faster to occur. Therefore, under high temperature conditions, the luminescence activity of DMAE-NHS will decrease faster. Understanding the relationship between the thermal stability of acridine ester DMAE-NHS and temperature, pH value, and hydrolysis reaction is of great significance for us to use it reasonably in practical applications. In practical operation, we can create a stable environment for DMAE-NHS as much as possible by controlling temperature and pH values, slowing down the occurrence of hydrolysis reactions, thereby extending its service life and fully utilizing its role in areas such as chemiluminescence detection. Hubei Xindesheng Material Technology Co., Ltd., as a chemiluminescence reagent manufacturer with nearly 20 years of R&D and production experience, has mature technology and rich experience in the research and production of acridine ester DMAE-NHS and other chemiluminescence reagents. We purchase products from Desheng and have a professional technical team to provide excellent customized and after-sales services. If you are interested in this type of product, please click on the official website to learn more details or contact me!    

In the fields of chemical, daily chemical, and industrial production, finding a raw material that combines efficient dissolution, strong chelating ability, and pH adjustment function is the key to improving product stability and reducing costs. Tetrapotassium EDTA, with its ultra-high solubility, strong alkalinity, and potent chelating properties, has become a "versatile" in many industries. Its value is being recognized by more enterprises, from laboratory mother liquor preparation to daily chemical product formula optimization. 1, Ultra high solubility: efficient configuration, worry free production The solubility of tetrapotassium EDTA is as high as ≥ 1500g/L, which gives it a significant advantage in the solution preparation process. Traditional chelating agents often cause uneven concentration of mother liquor, equipment blockage, and even affect subsequent production processes due to slow dissolution rates or residual issues. And EDTA tetrapotassium can achieve "instant dissolution without residue", even under high concentration preparation requirements, it can quickly form a uniform solution, greatly reducing preparation time. For production enterprises, this characteristic directly translates into efficiency improvement: there is no need to repeatedly stir or heat dissolve, reducing labor and energy consumption; High concentration mother liquor can be diluted as needed to avoid frequent replacement of raw materials and reduce storage costs; The residue free characteristic can better protect equipment pipelines, extend their service life, and reduce the risk of downtime and maintenance caused by blockage. Whether it is large-scale industrial production or small-scale laboratory preparation, the "ease of use" of EDTA potassium has become a key factor in improving production smoothness. 2, Strong alkalinity (pH 11-12): dual-use, cost optimization The pH value of a 1% aqueous solution of potassium EDTA reaches 11-12, which gives it unique value in the fields of daily chemical and cleaning due to its strong alkaline properties. In traditional formulas, chelating agents and alkaline modulators often need to be added separately, which not only increases the variety of raw materials but also increases the complexity of the formula. The "alkaline+chelating" dual function of EDTA tetrapotassium can simultaneously accomplish two tasks: In cleaning agents, their strong alkalinity can effectively decompose stubborn stains such as oil stains and soap deposits, while chelating calcium and magnesium ions in water to prevent hard water from interfering with the cleaning effect and improve the cleaning power; In daily chemical products such as shampoo and shower gel, EDTA tetrapotassium can chelate metal ions to prevent formula oxidation and discoloration, while adjusting pH to an appropriate range to reduce skin irritation and enhance product mildness. For formulators, the "dual-use" of EDTA tetrapotassium means reducing the procurement and addition steps of at least one raw material, simplifying supply chain management; For production enterprises, formula optimization directly reduces raw material costs and production energy consumption, and enhances product market competitiveness. 3, Strong chelation: Stability guardian, quality assurance The core value of EDTA tetrapotassium lies in its strong chelating ability, which can firmly bind various metal ions such as Ca ² ⁺, Mg ² ⁺, Fe ² ⁺/³ ⁺, etc. Metal ions are "invisible killers" that cause product deterioration, precipitation, oxidation, or efficiency decline - for example, calcium and magnesium ions in water can react with soap to form soap deposits, reducing cleaning effectiveness; Iron ions can catalyze the oxidation of oils and fats, causing food or cosmetics to become rancid and discolored. EDTA tetrapotassium "locks" these metal ions to form stable chelates, blocking their participation in adverse reactions from the source: in the food industry, it can extend shelf life and prevent discoloration and flavor change; In cosmetics, formula stability can be maintained to avoid layering or precipitation; In industrial water treatment, it can prevent the deposition of metal ions and protect equipment from corrosion. Since its establishment in 2005, Hubei Xindesheng has been deeply engaged in the field of biochemistry for 20 years, accumulating rich experience in product production and use. At present, our carefully crafted EDTA tetrapotassium has a wide range of applications, high purity, and can meet various usage scenarios. At the same time, the company has a large-scale production base with strong production capacity. Whether you want to experience quality in small quantities or meet production needs through large-scale procurement, we can provide timely supply. If you are interested in our products, please feel free to call us for consultation and ordering at any time, and start a new chapter of cooperation and win-win!  

In the field of medical testing, the quality of separating gel collection tubes directly affects the serum separation efficiency and detection accuracy. This article will systematically review the entire process of adding glue to the separation glue collection tube, from the amount of glue used, operational skills to special scenario handling, providing practical guidance for production personnel. Glue dosage and thickness control: precise proportioning is the foundation The gluing process must strictly follow the corresponding standards for pipe diameter and dosage: Φ 13mm pipe diameter: It is recommended to add 0.8-1g of adhesive to ensure that the adhesive layer thickness reaches 6mm; Φ 16mm pipe diameter: It is recommended to add 1.4-1.6g of adhesive, and also ensure a thickness of 6mm. Insufficient thickness of the adhesive layer can lead to mixing of serum and blood cells, affecting the detection results; Excess may increase production costs. In practical operation, precise control can be achieved through colloid filling equipment. If there are doubts about the dosage, sales personnel can be contacted for personalized solutions. Winter gluing: temperature management is key Under low temperature conditions, the separation gel is prone to difficult flipping due to hardening, which affects the quality of serum separation. To address this issue, the following measures need to be taken: Natural temperature recovery: After transferring the colloid from outdoors to a 25 ℃ laboratory, let it stand for 30 minutes to 1 hour until the colloid softens before operation; Water bath heating: If rapid heating is required, the colloid can be heated in a 25 ℃ water bath (not exceeding 4 hours) to promote the recovery of colloid fluidity. By regulating the temperature, it is possible to effectively avoid the problem of poor flipping caused by colloidal hardness, ensuring the efficiency of serum separation. Laboratory gluing: strategies for addressing wire drawing issues If there is a wire drawing phenomenon during the gluing process, it not only affects the operational efficiency, but also may contaminate the blood collection tube.  The solution is as follows: 1. Water bath pretreatment: Heat the colloid in a 25 ℃ water bath (not exceeding 4 hours) to reduce its viscosity; 2. Equipment debugging: Check whether the nozzle of the filling equipment is blocked or worn, and replace the accessories in a timely manner; 3. Operation optimization: Keep the test tube vertical when adding glue, control the filling speed, and avoid excessive friction between the glue and the tube wall. Static storage and transportation after adding glue: details determine success or failure After adding the adhesive, it needs to be left vertically for 1-3 minutes to restore the thixotropy of the colloid and form a stable adhesive layer. Then, it should be placed flat and enter the next process. During the subsequent production and storage process, attention should be paid to: Avoid continuous flat placement: Prolonged flat placement may cause colloid flow, affecting the separation effect; Prevent inversion: Inverting can cause the colloid to come into contact with the pipe plug, increasing the risk of contamination; Standard stacking: Use dedicated trays or brackets to fix test tubes and maintain a vertical position. Bubble elimination: centrifugal process optimization Bubbles are a common issue that affects the quality of blood collection tubes and need to be treated through centrifugation technology Centrifuge after vacuum plugging: It is recommended to leave the plug for 24 hours before centrifuging and packaging to ensure that the bubbles are fully visible; Avoid centrifugation before plugging: Pre plugging centrifugation may cause colloidal displacement. It is recommended to check for bubbles 24 hours after plugging and re centrifuge if necessary; Equipment selection: Prioritize the use of inclined centrifuges to improve defoaming efficiency. If the blood collection tube needs to be sterilized by irradiation, the dose of cobalt 60 should be controlled to be ≤ 25kGy to avoid damage to the colloidal properties. Hubei Xindesheng Material Technology Co., Ltd., as a professional enterprise in the field of blood collection tube additives such as serum separation gel, has always been committed to providing customers with high-quality products and comprehensive services. We not only have high-quality serum separation gel products, but also a full range of blood collection tube additives such as anticoagulants, coagulants, silicides, etc. If you have any related procurement needs in the near future, please click on the official website to learn more details!