Wuhan Desheng Biochemical Technology Co., Ltd

In recent years, the in vitro diagnostic (IVD) industry in China has encountered unprecedented development opportunities driven by multiple factors such as the continuous growth of global medical demand, technological progress, and population aging. By 2025, the size of China's IVD market is expected to exceed 135 billion yuan, becoming an indispensable and important component of the modern healthcare system. Industry status: Steady growth, diverse highlights in segmented fields The IVD industry in China has shown a steady growth trend in recent years. According to market research institutions, the size of China's IVD market has reached 125 billion yuan in 2024, a year-on-year increase of 8.5%. It is expected that by the end of 2025, the market size will exceed 135 billion yuan, with a growth rate maintained at around 8%. In the segmented field, immunodiagnosis still holds the largest market share, thanks to the continuous development of technologies such as chemiluminescence and electrochemiluminescence. Its applications in infectious diseases, tumor markers, thyroid function and other detection projects are becoming increasingly widespread. Molecular diagnostics, as the fastest growing sub field, has an average annual growth rate of about 20%. The continuous innovation of technologies such as PCR, NGS, dPCR, etc. has promoted their widespread application in infectious disease diagnosis, tumor gene testing, and other fields. The POCT market, with its fast and convenient characteristics, has a strong demand in primary healthcare, emergency treatment and other scenarios, and is expected to maintain a high growth rate in the coming years. Technological innovation: the core driving force for industry development Technological innovation is a key factor driving the development of the IVD industry. In the field of immunodiagnosis, the integration of chemiluminescence technology with microfluidics and AI has become an important trend, greatly improving the sensitivity and automation level of detection. In the field of molecular diagnostics, the continuous optimization and upgrading of PCR technology, especially the application of digital PCR (dPCR), has further improved the accuracy of detection, providing strong support for tumor liquid biopsy, rare disease gene detection, and so on. POCT technology is developing towards miniaturization, intelligence, and multi index joint inspection. By combining IoT and AI technology, it achieves real-time transmission and intelligent analysis of detection data, meeting the needs of primary healthcare, home self inspection, and other scenarios. Competitive landscape: Market reshuffle under diversified situation The competitive landscape of China's IVD industry is showing a diversified trend, with foreign-funded enterprises and local enterprises competing on the same stage. Foreign funded enterprises dominate the high-end market with advanced technology and brand advantages, but with the continuous breakthroughs in local enterprise technology and the improvement of product quality, their market share is gradually being squeezed. Local enterprises, relying on cost advantages, in-depth understanding of the local market, and policy support, occupy a large share in the mid to low end market and continuously penetrate into the high-end market. The implementation of centralized procurement policy and the promotion of medical insurance payment reform have accelerated market reshuffle, putting greater pressure on small and medium-sized enterprises to survive, and further concentrating market share towards top enterprises. Future trend: Emerging markets and international development become new growth points Looking ahead, the IVD industry in China will continue to maintain a stable growth trend. With the continuous growth of medical demand, the promotion of technological innovation, and the optimization of policy environment, the industry scale will continue to expand. Emerging markets such as pet medical testing and consumer health testing will become new growth points, bringing new development opportunities to the IVD industry. As an upstream IVD reagent raw material manufacturer, Hubei Xindesheng Company can supply a variety of raw materials suitable for molecular diagnostics and other fields, including biological buffering agents, luminescent reagents, enzyme reaction substrates, etc. Hubei Xindesheng Material Technology Co., Ltd. has always maintained the research and development production philosophy of "exploring and innovating, pursuing excellence" in the face of current challenges and opportunities. At the same time, it actively expands overseas markets, participates in international competition, and enhances its international market share.    

In biochemical experiments, CAPS buffer, as an important alkaline buffer, is widely used in Western blotting, enzyme catalyzed reactions, and HPLC separation due to its stable pKa value (about 10.4), good water solubility (up to 11.07 mg/mL at 25 ℃), and low cell membrane permeability. However, experimenters often find that the solubility of CAPS significantly decreases at low temperatures (such as 4 ℃ or -20 ℃), leading to difficulties in buffer preparation or uneven concentration, which in turn affects the reliability of experimental results. This article will analyze this phenomenon from three levels: molecular mechanisms, environmental factors, and experimental operations, and propose targeted optimization solutions. Molecular mechanism of low temperature solubility decrease The dissolution process of CAPS is essentially the process of its molecules forming a hydration layer with water molecules through hydrogen bonding. At room temperature, the sulfonic acid group (- SO3H) and amino group (- NH -) in CAPS molecules combine with water molecules through polar interactions to form stable solute solvent complexes. However, as the temperature decreases, the thermal motion of water molecules weakens, and the hydrogen bonding network tends to become rigid, resulting in a decrease in the binding energy between CAPS molecules and water molecules. Experimental data shows that the solubility of CAPS decreases by about 30% at 4 ℃ compared to 25 ℃, which is closely related to changes in the dynamic properties of water molecules. In addition, the crystallization behavior of CAPS undergoes significant changes at low temperatures. At room temperature, CAPS molecules are dispersed in a disordered state in solution; When the temperature drops below the critical point, molecules form an ordered lattice structure through hydrophobic interactions and π - π stacking. This phase transition process will further reduce the solubility of CAPS and even lead to the precipitation of undissolved solid particles. For example, when preparing CAPS buffer solution, if the solvent is not sufficiently preheated, white flocculent precipitates can often be observed, which is a direct manifestation of low-temperature induced crystallization. The synergistic effect of environmental factors on solubility In solvent ion strength experiments, deionized water is often used to prepare CAPS buffer solution. However, if there are residual metal ions (such as Ca ² ⁺, Mg ² ⁺) in the water, they will form complexes with the sulfonic acid groups in CAPS molecules, reducing their effective solubility. At low temperatures, the hydration of ions is enhanced, the stability of the complex is improved, and further exacerbating the decrease in solubility. For example, in a solution containing 0.1 mM Ca ² ⁺, the solubility of CAPS at 4 ℃ is reduced by 15% compared to pure water system. The solubility of CAPS is closely related to its dissociation state due to pH fluctuations. When the pH is below pKa (10.4), CAPS molecules exist in protonated form (- SO3H) with high solubility; When the pH approaches or exceeds pKa, the solubility of the deprotonated form (- SO ∝⁻) significantly decreases. Under low temperature conditions, the pH value of the buffer solution may drift due to the dissolution of CO ₂ or hydrolysis of impurities, indirectly affecting the dissolution behavior of CAPS. By understanding the molecular mechanism and environmental factors that contribute to the decrease in low-temperature solubility of CAPS, researchers can optimize the preparation process and storage conditions in a targeted manner to ensure the stability of the buffer performance. In the future, with the development of new zwitterionic buffering agents, the limitations of CAPS in low-temperature experiments are expected to be fundamentally resolved. As a professional biological buffering agent manufacturer, Desheng is committed to providing high-quality CAPS buffering agents. We not only have professional personnel to supervise and control the process from raw material use to factory preparation, but also continuously optimize testing methods to meet the diverse needs of our customers. There is stock available for sale in the warehouse at a cheap price. If you have any relevant intentions, please feel free to click on the website to inquire about details and purchase at any time!

In cell culture and biological experiments, HEPES buffer and phenol red indicator are often used simultaneously. The former is used to maintain pH stability in the culture medium, while the latter is used to visually display pH changes. However, the interaction between the two in terms of chemical properties may lead to color abnormalities, affecting the accuracy of experimental results. This article will analyze the causes of this phenomenon and provide simplified optimization solutions. Chemical basis of color interference Phenol red is a pH sensitive dye that changes color with acidity or alkalinity: it appears yellow in acidic environments (pH8.2). This characteristic makes it an ideal tool for monitoring the pH of cell culture media. The core function of HEPES as a buffer is to stabilize pH by releasing or absorbing hydrogen ions. The problem is that the sulfonic acid groups in HEPES molecules have a similar chemical structure to phenol red, and when the concentration of HEPES is high, they will interact with phenol red to change its molecular structure. This change causes the color of phenol red to become lighter or shift at a specific pH, for example, it may appear orange red instead of standard red at pH 7.4. The intuitive manifestation of interference phenomenon In conventional cell culture, if high concentrations of HEPES (such as over 20mmol/L) and phenol red are used simultaneously, the color of the culture medium may be lighter or yellowish than expected. For example, the pH 7.4 culture medium, which should have displayed red, may have turned pale orange due to HEPES interference, leading researchers to misjudge the pH value. In fluorescence microscopy observation, this interference is more pronounced. Phenol red emits fluorescence at specific wavelengths, while HEPES may absorb some of the fluorescence signal, resulting in decreased image brightness or increased background noise. This effect is particularly prominent in long-term observation or live cell imaging experiments, which may mask the true state of cells. Simplified optimization plan Adjust HEPES concentration 1. Conventional cultivation: Control the concentration of HEPES within 10-15 mmol/L, which has minimal interference with the color development of phenol red and can effectively maintain pH stability. 2. Short term experiment: If the experimental time is short (such as

In the use of the new Trinder's reagent TODB, high background is a common problem that affects the detection results, while insufficient sealing and incomplete plate washing are key factors that are easily overlooked during operation. These two issues can cause an abnormal increase in the color background due to non-specific binding and interference from residual substances. Below is a detailed analysis and solution. Inadequate closure: the 'behind the scenes' driving force behind non-specific combinations The function of the blocking step is to block the non binding sites on the surface of the reaction carrier (such as an enzyme-linked immunosorbent assay plate). If the sealing is not sufficient, the substrates, enzyme conjugates, and other components in the TODB reagent will randomly adsorb onto the surface of the carrier, forming a color signal without the participation of the target substance, directly increasing the background value. specific reason 1. Insufficient sealing time: Generally speaking, sealing requires placing at 37 ℃ for 60 minutes or at room temperature for 120 minutes. If the time is shortened to less than 30 minutes, the active sites on the surface of the carrier cannot be completely covered by the blocking solution (such as BSA, skim milk powder), and those hydrophobic areas that are not blocked will actively adsorb protein components in the TODB reaction system, resulting in background coloration. 2. Too low concentration of blocking solution: When the effective ingredients in the blocking solution are insufficient, such as when the original 5% BSA drops to 1%, a tight protective film cannot be formed between molecules. Components such as horseradish peroxidase in TODB reagent will stick to the inner wall of the plate pore through hydrophobic interactions, and non-specific reactions will occur with the substrate during the reaction. 3. Failure of sealing solution: If the sealing solution is repeatedly frozen and melted, or stored for more than its expiration date, the protein components inside will deteriorate and lose their sealing function, resulting in many "exposed" sites on the surface of the carrier, which become the source of background signals. Incomplete board washing: the "stacking effect" of residual substances The main function of plate washing is to remove unbound free reagents (such as unbound antibodies, TODB precursor substances). If the plate washing is not thorough, residual substances will continue to participate in color development in subsequent reactions, allowing background interference to accumulate. Specific Reason 1. Too few plate washes: Conventional testing requires washing the plate 3-5 times. If it is reduced to less than 2 times, the residual free enzyme complexes in the well cannot be completely removed, and the reaction will react with the TODB substrate, resulting in additional color development. 2. Too much detergent residue: After washing the board, if the holes are not inverted on the absorbent paper and patted dry, there will be more detergent residue in each hole, and some components inside will disrupt the balance of the TODB reaction system. At the same time, residual enzyme conjugates will diffuse into the adjacent holes with the liquid, causing cross contamination and raising the background. 3. There is a problem with the washing machine: When the needle of the washing machine is blocked or the pressure is insufficient, the corners of the plate holes will become areas that cannot be washed. The residual TODB reagent will crystallize after drying and participate in the reaction when dissolved again, causing the local background color to darken. Summary: Operational details determine the effectiveness of background control Although sealing and washing plates are routine steps, their quality directly affects the background level of TODB reagents. In practical use, it is recommended to adopt the method of "extending the sealing time+increasing the number of plate washes", combined with measures such as checking the concentration of the sealing solution and regularly calibrating the washing machine, which can significantly reduce the background value and provide assurance for the accuracy of the detection results. Desheng specializes in producing more than ten new Trinder's reagents, including TODB. After more than ten years of research and development, it can ensure that TODB appears as a powder with a purity of up to 99.5%, strong water solubility, and stable performance to ensure the accuracy of experimental results. Desheng has a place in the market for in vitro diagnostic kit raw materials with high-quality products, and is deeply trusted and supported by customers at home and abroad. If you have any relevant intentions, please click on the official website for consultation!